Based on the samples from the west wall of the ABYT2004 at Yuhuicun Site, the environmental substitute indexes such as the grain-size, Rb/Sr, heavy-mineral (Zircon) and magnetic susceptibility were analyzed. The age of archaeological stratigraphy was determined by the AMS14C. The results reveal that the environment evolution induced to the climate changes from 4.5kaBP to 4.0kaBP, Longshan Culture Period. Besides, the information of the ancient human activities and the cultural heritage data withdrawn from the Longshan Culture archaeological stratigraphy, shows that the Longshan culture development was an influenced mechanism. Archaeological excavation shows the following in the study area: (1) In the period before 4500aBP, from 4500aBP to the middle of the period of Longshan Culture, to the later period of Longshan Culture and to 4000aBP, most parts of the region experienced climate change such as warm and wet, cold and dry, warm and wet, cold and dry. (2) Before 4500aBP of the wet period, the Huaihe River water reached a very high level so that the Yuhuicun Site and the surrounding area were submerged and people could not live in the area around the site. From 4500aBP of the dry period, the water level in the Huaihe River and its branches started to descend. (3) Around 4100aBP, in the later period of Longshan culture, it again became cold and dry, and the river water flooded the land again. During this period, the agricultural activity was destroyed, and Longshan Culture disappeared. (4) The environmental information in sedimentary strata and the historical records from the literature confirmed each other, which reveals the close relationship between the Yuhuicun Site and the "Dayu Controlling Flood" recorded in historical documents.